Pronuclei Formation and Early Development of Human Oocytes after Intracytoplasmic Sperm Injection according to Maturity and Motility of Spermatozoa Geun-Joo Kim, Jong-Heung Kim, Sang-Chan Lee, and Byung-Ki Kim
Dev. Reprod. Vol. 3, No. 1, 39-44, 1999
Pronuclei Formation and Early Development of Human Oocytes afrer
Intracytoplasmic Sperm Injection according to Maturity an Motility of
Spermatozoa
Geun-Joo Kim, Jong-Heung Kim*, Sang-Chan Lee* and Byung-Ki Kim
Department of biology, college of Natural Science, Dongeui University,
Pusan, 614-714, Korea
*Saewha Infertility Institute, Pusan, Korea
ABSTRACT
In the present study, we investigated the effect of maturity of
spermatozoa on the formation of pronucleus and subsequent developmental
capacity of human embryo in vitro. The fertilization was perfored by means
of intracytoplasmic sperm injection (ICSI) in HEPES-buffered m-TCM-199
medium. In the first of the experiment, motile or immotile human
spermatozoa ejaculated were injected into cumulus-enclosed human oocyte
matured in vivo. Significantly(p<0.002) higher proportion of oocytes that
was injected with motile spermatozoa formed 2 pronuclei than the oocytes
injected with immotile spermatozoa (79.8% vs 51.7%). In the second part of
the experiment, cumulus-enclosed human oocytes maturd in vivo were
injected with motile or immotile spermatozoa collected form testes. There
was no difference between motile and immotile spermatozoa. In third part
of the experiment, using modified Tyrode's medium containing 10.0 mM
lactate, 0.5mM pyruvate, 0.2 mM taurin, 1.0mM glutamine, 2.22 mM MEM amino
acids, vitimin and 10% human follicular fluid, we found that the
development of oocytes that formed 2 pronuclei were able to develop to 9-
16 cells regardless of maturity and motility of spermatozoa.
key words: Intracytoplasmic sperm injection, Ejaculated spermatozoa,
Testicular spermatozoa, Pronuclei, In vitro development.