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Effect of the presence of glucose during fertilization and/or culture in a chemically semi-defined medium on the development of bovine oocytes matured and fertilized in vitro. Jeong-Mook LIM, Jong-Heung Kim, Kiyoshi OKUDA and Koji NIWA 

​Journal of Reproduction and Development, Vol. 39, No. 3, 1993 

Effect of the presence of Glucose during Fertilization and/or

Culture in a Chemically Semi-Defined Medium on the Development

of Bovine Oocytes Matured and Fertilized in Vitro

Jeong-Mook LIM, Jong-Heung Kim, Kiyoshi OKUDA and Koji NIWA

Division of Animal Science and Technology, Faculty of Agriculture, Okayama 

University, Okayama 700, Japan

Abstract

Bovine cumulus-oocyte complexes were inseminated in fertilization medium, 

mBO medium including caffeine(5mM), heparin(10μg/ml) and bovine serum 

albumin(BSA; 10mg/ml) with or without glucose(13.9mM). Oocytes were freed 

from cumulus cells 8h post-insemination and transferred into culture 

medium, mTCM-199 supplemented with BSA(3mg/ml) and with or without glucose

(5.56mM). When oocytes were examined 24 and 72h post-insemination, high 

proportions of oocytes were penetrated(88-92%) and cleaved to more than 

the 2-cell stage(68-71%), respectively, regardless of the presence or 

absence of glucose during fertilization and/or further culture without the 

morula and blastocyst stages, respectively, were significantly(P<0.05) 

higher proportions of oocytes developed to the morula(23 vs. 40%) and 

blastocyst(5 vs. 12%) stages when they were cultured in glucose-free 

medium after fertilization in medium without than with glucose. When 

glucose was added in culture medium 120 or 144h post-insemination, 23 to 

24% of inseminated oocytes developed to the early or expanded blastocyst 

stage 192h post-insemination.

key words: Bovine, OOcytes, In vitro fertilization, Embryo development, 

Glucose.