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​사람의 과배란 유도 후 난소 반응별 난포액 내 단백질 변화​ 

Reproductive & Developmental Biology Vol.35(3) : 273-280(2011)

The Change of Protein Patterns in Follicular Fluid on Ovarian Response

Following Controlled Ovarian Hyperstimulation (COH) of Human

(사람의 과배란 유도 후 난소 반응별 난포액 내 단백질 변화)

Chae-Sik Lee1, Sang-Chan Lee1, Yong-Ho Rho1, Dae-Sik Oh1, Yong-Seung Lee2, Eun-Ji Song2,

Hee-Tae Cheong2, Boo-Keun Yang1 and Choon-Keun Park2,†

(이채식1․이상찬1․노용호1․오대식1․이용승2․송은지2․정희태3․양부근2․박춘근2,†

1세화병원 난임연구소,2강원대학교 동물생명과학대학,3강원대학교 수의과대학)

1Infertility Institute, Saewha Hospital, Busan 607-843, Korea

2College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea

3School of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Korea

ABSTRACT

detect the change of protein patterns in follicular fluid on ovarian response following controlled ovarian hyperstimulation for human IVF outcome. In the normal group, it was confirmed reproducible 57 spots in the detected total 81spots. Then 1 spot was not found in the other groups. In the poor responder group, it was found reproducible 53spots in the detected total 98 spots. 6 spots were down-regulation and 7 spots were up-regulation comparable with normal group. There were not 5 spots in poor responder group comparable with other groups. In the polycystic ovary syndrome group, it was expressed reproducible 53 spots in the detected total 80 spots and 3 spots were just expressed in this group. However, 4 spots were not found in polycystic ovary syndrome. 9 spots were up-regulation comparable with normal group. Significant up and down-regulation spots among the each groups were identified. The results were

a cytosolic carboxypeptidase, a signal-induced proliferation-associated protein 1, a ceruloplasmin, a keratin(type Ⅱcytoskeletal 1), a polypeptide N-acetylgalactosaminyltransferase 2, a serine/threonine-protein phosphatase 4 regulatory subunit 4. It was identified that 8 spots, 6 kinds of protein are corresponded with NCBInr database research, but 10spots were failed in the identification. In conclusion, it has been confirmed change and expression of protein on the ovarian response following COH of human.

(Key words : 2-DE, COH, Follicular fluid, Portein, Ovarian response, Human IVF)

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