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Changes of Glycosidase Activity of Frozen-Thawed S​

Reproductive & Developmental Biology Vol.35(2) : 185-190(2011)

Changes of Glycosidase Activity of Frozen-Thawed Spermatozoa in Human

(인간정자의 동결, 융해 후 Glycosidase 활성의 변화)

Chae-Sik Lee1,3, Sang-Chan Lee3, Ji-Eun Lee1, Hee-Tae Cheong2, Boo-Keun Yang1 and Choon-Keun Park1,†

1College of Animal Life Sciences, Kangwon National University, Chuncheon 200-701, Korea

2College of Veterinary Medicine, Kangwon National University, Chuncheon 200-701, Korea

3Saewha Women’s Clinic, Busan 607-060, Korea

Abstract 

To evaluate the effect of spermatozoa culture on glycosidase activity of frozen-thawed spermatozoa in human, the spermatozoa were treated experimentally and assayed for activities of α-L-fucosidase, α-D-mannosidase, β-D-galactosidase and N-acetyl-β-D-glucosaminidase (β-GlcNAc’ase). The β-GlcNAc’ase activity was at least two-folds higher than other glycosidases regardless of spermatozoa incubation (p<0.05). The spermatozoa motility was decreased with incubation periods, but no effects by different glycosidases on the changes of spermatozoa motility during the various periods of incubation. In all glycosidases, the spermatozoa-zona binding rates in spermatozoa without incubation were higher than in spermatozoa incubated for 2 h (p<0.05). β-GlcNAc’ase is present mainly in the plasma membrane of spermatozoa frozen-thawed in human. It was also shown that the glycosidase activity was increased in all 

glycosidases in spite of lower sperm-zona binding by spermatozoa incubation.

  (Key words : Acrosome reaction, Glycosidase activity, In vitro, Human, Spermatozoa incubation)

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